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. 2021 Feb 11;16:1021–1036. doi: 10.2147/IJN.S283406

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© 2021 Hu et al.

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Osteogenic and adipogenic differentiation of PDLSCs on different titanium surfaces. (A) The relative mRNA levels of RUNX2 and ALP in PDLSCs cultured on different surfaces for 7 days with osteoinduction. (B) (Left panel) Immunofluorescence staining assay for RUNX2 in PDLSCs (red, RUNX2; blue, DAPI) cultured on different surfaces for 7 days. (Right panel) Immunofluorescence stain intensity analysis of RUNX2. (C) Total protein expression of RUNX2 and ALP in PDLSCs at 7 days after osteoinduction. (D) ALP activity of PDLSCs cultured on Ti, SLA, and SLM-AHT titanium surfaces for 7 days. (E) The relative mRNA levels of PPARγ and C/EBPα in PDLSCs cultured in the adipogenic conditions on different titanium surfaces for 7 days. (F) The total protein expression of PPARγ in PDLSCs at 7 days after adipoinduction was examined by Western blot. Error bars represent SD (n = 3). **P < 0.01,***P < 0.001 .