Figure 2.

(A) Molecular structure of R-chymotrypsin. (B) Schematic representation of monolayer-controlled diffusion of the substrate into and the product away from the active pocket of nanoparticle bound ChT. (C) Chemical structure of amino-acid-functionalized gold nanoparticles and SPNA-derived substrates. (D) Generation rate (V) of 4-nitroaniline from different substrates under catalysis by ChT or ChT-nanoparticle complex. Inset shows the normalized activity of ChT-NP complex to ChT toward different substrates. Reprinted with permission from You CC, Agasti SS, De M et al Modulation of the catalytic behavior of α-Chymotrypsin at monolayer-protected nanoparticle surfaces. J Am Chem Soc. 2006;128(45):14612–14618. Copyright (2006) American Chemical Society.²³