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. 2021 Feb 16;4:211. doi: 10.1038/s42003-021-01728-8

Fig. 6. Use of mCherry-GAL9 reporter line with automated particle formulation.

Fig. 6

a Overview of experimental approach. particles are formed by automated pipetting in a plate-based format and feed directly into plate-based assays for particle size determination by dynamic light scattering (DLS), encapsulation by ribogreen integration and for dosing mCherry-GAL9 reporter cells directly. b Heatmap summary of LNP formulation variation data representing normalised Cy5-mRNA uptake, mCherry-GAL9 puncta, EGFP fluorescence and % EGFP positive cell values at 14 h post-dosing. Red square indicates standard composition of particles used in rest of manuscript. c–e Overview of particle characteristics and kinetic results obtained with the screening approach looking at comparisons of particles based upon variable components. Values plotted are mean normalised values ± SEM c Sterol modified particles, n = 24 LNPs per group from n = 4 independent experiments. d Ionisable lipid modified, only data with cholesterol particles shown, n = 8 per group from n = 4 independent experiments e PEG-lipid modified, only data with cholesterol particles shown, n = 12 per group from n = 4 independent experiments.