Fig. 1. Assessment of antibody in vivo protection.

a Parasite liver burden load was measured by bioluminescence of P. berghei sporozoites expressing luciferase-conjugated PfCSP after passive transfer of 100 µg of antibody in C57Bl/6 mice (N = 5). The numbers indicate the percent inhibition of the mean parasite burden relative to that of naïve control mice (i.e., % inhibition). A two-sided Mann-Whitney U test was used; *p = 0.0159, and **p = 0.0079. The bar denotes the geometric mean, and error bars denote the SD. Red dashed lines indicate the baseline signal from naïve non-infected mice treated with D-luciferin as established previously³². Parasite-free mice after passive immunization with b 300 µg or c 100 µg of the indicated antibodies before challenge with bites of infected mosquitoes. A log-rank test was used; in b *p = 0.032 (mAb395), **p = 0.0006 (mAb239, 356, and 364), 0.0005 (mAb311; second panel, and mAb317), 0.0013 (mAb399 and 224), 0.0022 (mAb311, first panel), 0.0053 (mAb337), and 0.0089 (mAb366 and 397); in (c) *p = 0.019 (mAb399 and 366), **p = 0.0051 (mAb239, 356, 364, 224, and 317) (N = 6 per group). Source data are provided as a Source Data file.