Table 1.

Dissociation constants of antibody Fabs and fold-changes in apparent affinity obtained from ITC.

mAb	Kd to NPNA2 (nM)	Kd to NPNA4 (nM)	Kd to NPNA6 (nM)	Mean Kd fold-change NPNA2 vs NPNA6
239	641 ± 38	20 ± 2	10 ± 1	64
311	152 ± 11	14 ± 5	19 ± 4	8
337	1523 ± 11	71 ± 1	59 ± 14	26
356	8272 ± 1284	82 ± 18	30 ± 6	276
364	47 ± 2	28 ± 2	27 ± 3	2
395	6152 ± 196	1423 ± 404	999 ± 231	6
mAb	Kd to NPNA2 (nM)	Kd to NPNA4 (nM)	Kd to NPNA6 (nM)	Mean Kd fold-change NPNA4 vs. NPNA6a
224	14245 ± 4507	126 ± 14	141 ± 8	1
366	1348 ± 110	1770 ± 82	479 ± 17	4
397	2743 ± 156	255 ± 10	92 ± 8	3
399	13345 ± 2017	5922 ± 1122	94 ± 30	63
317	132 ± 62	70 ± 12	111 ± 23	1

^aThe fold-change calculation for these two sets of antibodies is between NPNA₄ and NPNA₆ as it was shown by the crystal structures that their minimal epitopes are NPNA₃. Thus, the comparison between NPNA₂ and NPNA₆ would not be appropriate here.