Table 2.
Dissociation and rate constants of antibody Fabs to rsCSP obtained from bio-layer interferometry (BLI) displayed with mean dissociation constants measured to the longest peptide tested by isothermal titration calorimetry (ITC), mean % inhibition of parasite burden studies, % blood-stage parasite free mice from parasitemia studies, total paratope buried surface area (BSA), number of hydrogen bonds between paratope and epitope, and melting temperature (Tm) from differential titration calorimetry.
| mAb | Kd (nM) (ITC, NPNA6) | Kd (nM) (BLI, rsCSP) | kon (1/M ∙ s) | koff (1/s) | LB study 1 | LB study 2 | Parasit.study 1 | Parasit.study 2 | BSA (Å2) | # H-bond | Tm (˚C) | Epitope secondary structure | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| β-turn | 310 turn | ||||||||||||
| 317 | 111 | 9.3 | 2.32 × 104 | 2.15 × 10−4 | 94.0 | 100.0% | 519 | 7 | 73.3 | 3 | – | ||
| 224 | 141 | 16 | 5.69 × 104 | 8.97 × 10−4 | 96.0 | 83.3% | 617 | 10 | 68.9 | 3 | – | ||
| 399 | 94 | 26 | 6.77 × 104 | 1.78 × 10−3 | 94.3 | 83.3% | 492 | 7 | 82.0 | 2 | 1 | ||
| 311 | 19 | 31 | 3.83 × 104 | 1.19 × 10−3 | 96.5 | 93.4 | 83.3% | 83.3% | 473 | 7 | 71.3 | 1 | 1 |
| 364 | 27 | 32 | 4.53 × 104 | 1.45 × 10−3 | 93.9 | 100.0% | 443 | 6 | 72.1 | 1 | 1 | ||
| 356 | 30 | 42 | 3.89 × 104 | 1.62 × 10−3 | 94.1 | 83.3% | 572 | 6 | 74.4 | 1 | 1 | ||
| 239 | 10 | 187 | 2.93 × 104 | 5.48 × 10−3 | 94.0 | 83.3% | 550 | 8 | 71.8 | 1 | 1 | ||
| 397 | 92 | 63 | 4.92 × 104 | 3.10 × 10−3 | 83.3 | 50.0% | 593 | 10 | 74.4 | 1 | 1 | ||
| 337 | 59 | 97 | 5.41 × 104 | 5.22 × 10−3 | 80 | 66.6% | N/A | N/A | 85.2 | N/A | N/A | ||
| 366 | 479 | 1076 | 2.57 × 104 | 2.76 × 10−2 | 77.8 | 66.6% | 576 | 13 | 81.3 | – | – | ||
| 395 | 999 | 4902 | 3.96 × 104 | 1.94 × 10−1 | 31.3 | 50.0% | 420 | 7 | 80.3 | 1 | – | ||