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. 2021 Feb 16;11:3881. doi: 10.1038/s41598-021-82966-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of genes/proteins related to cholesterol efflux in bone marrow-derived macrophages and foam cells. Real-time RT-PCRs were performed using TaqMan probes specific for Abca1 (A), Abcg1 (B), ApoE (F), Hmgcr (G), Cd36 (H) and Sr-b1 (I) cDNAs. Units are the relative gene expression normalized to Hmbs. Most results showed expected differences between macrophages and foam cells but not between the genotypes. Results are shown as means ± SEM. p-values were determined by Student’s t-test, * p < 0.05. (C) Representative western blot images showing the levels of both ABCA1 and ABCG1 expression (red signal) in foam cells (FC) as compared to macrophages (Macro). β-actin (green) served as loading control. Data points represent individual mice from which bone marrow-derived macrophages were isolated and used for experiments. (D) Immunofluorescent detection (green signal) of ABCA1 and ABCG1 on macrophages (Macro) and foam cells (FC) derived from Abcc6^−/−;Ldlr^−/− and control Ldlr^−/− mice. Five representative images from each condition are shown. We only observed some staining pattern variation for ABCA1 in macrophages from Abcc6^−/−;Ldlr^−/− mice which appeared punctated as compared to cells from control Ldlr^−/− mice. (E) Negative controls for the immunofluorescent staining shows the specificity of the primary antibodies used. Nuclei were stained with DAPI.