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. 2021 Feb 16;11:3881. doi: 10.1038/s41598-021-82966-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Plasma bile acid profiles in Ldlr^−/− mice lacking Abcc6. Littermate mice were maintained on an atherogenic diet (high fat) or regular chow for 16 weeks. Panel (A) shows the primary bile acids: cholic, α- and β-muricholic acids and chenodeoxycholic acid. Panel (B) illustrates results for the secondary bile acids deoxycholic acid, ω-muricholic acid and hyodeoxycholic acid. Panel (C) represents data for the tertiary ursodeoxycholic acid. Results are shown as means ± SEM. p-values were determined by Student’s t-test. ****p < 0.0001.