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. 2021 Feb 3;14:616559. doi: 10.3389/fnins.2020.616559

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Copyright © 2021 He, Zhao, Wu, Cheng, Sun, Zhang, Yang, Fan, Di and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The detection of inflammatory cytokines through ELISA in vivo. (A,B) TNF-α and IL-1β levels were determined by ELISA after the intracerebroventricular injection of the S100A8 protein. (C,D) The pro-inflammatory cytokine production in the sham group at 24 h post-TBI, and the TAK-242 treatment of TBI mice. The data was presented as mean ± standard deviation (SD; n = 3, per group). ^∗∗P < 0.01 and ^∗∗∗P < 0.001, compared to the sham group; ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001, when compared to the S100A8 or TBI group.