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. 2021 Feb 3;11:634819. doi: 10.3389/fpls.2020.634819

FIGURE 3.

FIGURE 3

Overexpression of four MSTRG18363 variants and artificially synthesized form of MSTRG18363 (asf-MSTRG18363, consensus sequence of four MSTRG18363 variants) increased the ability of transgenic tomato plants to inhibit the infection of Botrytis cinerea. Four-week-old tomato plants were used to perform in vitro (pathogenicity tests on detached leaves) and in vivo (whole plant inoculation tests) assays. (A) qPCR analyses of MSTRG18363 expression in transgenic tomato plants. Phenotypes (B) and lesion diameters (C) of detached leaves from the MSTRG18363-overexpressing plants at 5 dpi. In the whole-plant inoculation tests, the leaves were sprayed with 2 × 105 spore mL− 1 spore suspension of B. cinerea. Disease index (D) and the expression of BcActin (E) were analyzed at 5 dpi. Different letters represented significant difference using Duncan’s multiple range tests at P < 0.05 for one way ANOVA.