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. 2021 Feb 3;11:634819. doi: 10.3389/fpls.2020.634819

FIGURE 6.

FIGURE 6

Bacillus subtilis SL18r-induced expression of MSTRG18363 functioned to mediate the SlATL20 gene by decoying miR1918. (A) Predicted base-pairing interactions between miR1918 and the binding site of MSTRG18363 with the designed base mutation. Red letters indicated mutated bases. (B) qPCR analysis of MSTRG18363, miR1918 and SlATL20 in the control, OE-asf-MSTRG18363, and OE-masf-MSTRG18363 tomato plants. (C) MSTRG18363 suppressed the expression of miR1918 in Nicotiana systems. The transcription of the miR1918 after the Agrobacterium strain harboring pCAMBIA1300-asf-MSTRG18363 and -masf- MSTRG18363 were introduced into the tobacco leaves that overexpressed miR1918. (D) The transcription of SlATL20 in both the TRV::00 and TRV::SlATL20 tomato leaves. (E) Phenotypes of detached leaves from the TRV::00, SL18r-inoculated and TRV::SlATL20 plants, and from the miR1918-overexpressing lines and TRV::SlATL20 plants inoculated with SL18r at 5 dpi. (F) Lesion diameters of the detached leaves. Moreover, in the whole-plant inoculation tests, the leaves were sprayed with 2 × 105 spore mL− 1 spore suspension of Botrytis cinerea. (G) Disease index and (H) B. cinerea actin gene expression of above the plants was detected at 5 dpi. Different letters represented significant difference using Duncan’s multiple range tests at P < 0.05 for one way ANOVA. Significant difference was analyzed using Tukey’s post hoc (**P < 0.01) or Duncan’s multiple range tests (different letters indicated statistically different at P < 0.05) for one way ANOVA.