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. 2021 Feb 15;9(2):e001608. doi: 10.1136/jitc-2020-001608

Figure 1.

Figure 1

Generation of allogeneic ‘off-the-shelf’ EBV-specific T cells using AdE1-LMPoly. (A) Schematic representing the process for manufacturing the ‘off-the shelf’ allogeneic EBV-specific T-cell bank (TIG-001–006) using PBMC isolated from seropositive healthy donors. AdE1-LMPpoly vector was used to infect 30% of the PBMC (MOI of 10:1), which were then irradiated and cocultured with the remaining PBMC for 2 weeks. These T-cell cultures were supplemented every 2–3 days with growth medium containing recombinant IL-2. On the 14th day, T cells were cryopreserved and assessed for EBV-specific reactivity. (B) T cells were stimulated with a peptide pool containing EBNA1, LMP1 and LMP2 peptide epitopes and then assessed for intracellular IFN-γ expression. Representative flow cytometry plots show the percentage of CD8+ T cells demonstrating EBV epitope-specific reactivity in TIG-001-006. EBNA1, EBV-encoded nuclear antigen 1; EBV, Epstein-Barr virus; IFN-γ, interferon-γ; IL-2, interleukin 2; LMP1, latent membrane protein 1; LMP2, latent membrane protein 1; PBMC, peripheral blood mononuclear cell.