Figure 6.

Assessment of therapeutic efficacy of allogeneic EBV-specific T cells in humanized mice-bearing EBV-positive B-cell lymphoma. (A) Schematic showing the reconstitution of the human immune system over 12 weeks in NRG mice using CD34⁺ cells. These mice were intravenously infected with EBV (B95.8 strain), infused with HLA-matched EBV-specific T cells on days 13, 17 and 21 post EBV infection and sacrificed 2 weeks after T-cell therapy. (B) Representative gross morphology of spleens illustrating the size and presence of lymphoid malignancies in the spleen (n=5 mice/group). The first group was mock treated with PBS. Among the treated groups, the second group was infused with three consecutive doses (at intervals of 96 hours) of TIG-005 T cells while the third group initially received two doses of TIG-005 and was later switched to TIG-002 (n=5 mice/group). (C–G) Spleen weight, overall viable cells, EBV load, viable CD45⁺CD19⁺ cells and viable CD19⁺CD23⁺ cells in spleen cells. The statistical significance of tumor weight data was determined using one-way ANOVA: *p<0.05, **p<0.01; ***p<0.001; ****p<0.0001. ANOVA, analysis of variance; EBV, Epstein-Barr virus; NRG, NOD-Rag1^null IL2rg^null.