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. 2021 Feb 15;9(2):e001749. doi: 10.1136/jitc-2020-001749

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See https://creativecommons.org/licenses/by/4.0/.

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CCR8 upregulation is induced by TCR stimulation. (A) UMAP plots showing the activity of the Foxp3, Cebpb, Prdm1, Bcl3 and Nfkb2 regulons within the distinct T-cell subsets, cells in which the regulons are active are indicated in blue. (B) ChIP-seq (p65) signal profiles across the Ccr8 locus. Data obtained from Oh et al.42 Peaks that are gained after Treg stimulation are highlighted (gray). (C) Percentage CCR8⁺ cells within C57BL/6 or OT-II splenic Tregs after 24 hours of in vitro co-culture with TCR stimulants (anti-CD3 +anti-CD28) or Chicken ovalbumin (OVA) (n=3). (D) Percentage CCR8⁺ cells within C57BL/6 or OT-II LN-derived Tregs after 24 hours of in vitro co-culture with TCR stimulants (anti-CD3+anti-CD28) or Chicken ovalbumin (OVA) (n=3). (E) Percentage CCR8⁺ cells within C57BL/6 splenic Tregs after 24 hours of in vitro co-culture with TCR stimulants (anti-CD3+anti-CD28) and distinct concentrations of CAPE (n=4). (F) Percentage CCR8⁺ cells within transferred C57BL/6 or OT-II splenic Tregs 48 hours after intratumoral (i.t.) adoptive transfer into LLC or LLC-OVA tumors (n=3). (C to F) Data shown as mean±SEM. (C, D) ***p<0.001 and ****p<0.0001 by one-way ANOVA where each condition was compared with prior to culture, (E, F) *p<0.05 and **p<0.01 by one-way ANOVA. ANOVA, analysis of variance; LLC, Lewis Lung Carcinoma; UMAP, Uniform Manifold Approximation and Projection.