Figure 3.

STAT1 is phosphorylated by c-Abl

(A) The immunoprecipitates prepared from 293T cells expressing His-STAT1 or His only were incubated with 10 ng of human Abl protein (Merck, 14–529) in the presence of 0.2 mM ATP. The reaction products were analyzed with the indicated antibodies.

(B and C) Anti-Flag or IgG immunoprecipitates prepared from 293T cells transfected with the indicated plasmids were analyzed by immunoblotting.

(D and E) Lysates of MCF-7 cells subjected to the indicated treatment were analyzed by immunoblotting.

(F) Lysates from JAK2-deficient or wild-type MCF-7 cells expressing the indicated plasmids were analyzed by immunoblotting.

(G) Wild-type or c-abl/arg-knockout MEFs were stimulated with IFNγ at 40 ng/mL for the indicated time (left) or with IFNγ at the indicated concentrations for 120 min (right). Lysates were analyzed by immunoblotting.