Figure 5.

c-Abl regulates IFN-related gene expression

The mRNA transcript level of IFN-induced genes was analyzed by RT-PCR. IFN-induced gene expression was measured by the ratio of specific gene transcription with IFN treatment to that without IFN treatment.

(A) Wild-type or c-abl/arg-knockout MCF-7 cells were treated with 40 ng/mL IFNγ for 6 hr

(B and C) MCF-7 cells were treated with or without 5 μM AMN107 for 24 hr and then subjected to 40 ng/mL IFNγ (B) or 1000 U/ml IFNα (C) stimulation for 6 hr. All quantitative data are shown as the mean ± SD of three technical experiments (unpaired Student's t-test). ∗∗p < 0.01.