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. 2021 Feb 5;2(1):100337. doi: 10.1016/j.xpro.2021.100337
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Mouse CD3-PerCP BioLegend Cat # 100326; RRID: AB_893319
Mouse CD45-APC-Cy7 BioLegend Cat # 103116; RRID: AB_312981
Mouse NK1.1-PE BioLegend Cat # 108708; RRID: AB_313395
Mouse Ly6G-BV421 BioLegend Cat # 127628; RRID: AB_256256

Bacterial and virus strains

Klebsiella pneumoniae Tsai et al., 2014 Klebsiella pneumoniae NVT1001, capsular serotype 1

Chemicals, peptides, and recombinant proteins

LB broth Alpha Biosciences Cat # L12-112;
Agar Gene Teks GT-PA001
DMEM-low glucose GIBCO-Thermo Fisher Scientific Cat#11885076
FBS Hyclone-Thermo Fisher Scientific SH30406.02
Penicillin streptomycin GIBCO-Thermo Fisher Scientific Cat#15140122
L-Glutamine GIBCO-Thermo Fisher Scientific Cat#A2916801
0.25% Trypsin-EDTA GIBCO-Thermo Fisher Scientific Cat#25200072
PBS pH 7.4 (1×) GIBCO-Thermo Fisher Scientific Cat#10010023
Fixation Buffer eBioscience-Thermo Fisher Scientific Cat# 00822249

Experimental models: cell lines

Primary human placental mesenchymal stem cells (PMSCs) Yen et al., 2005 n/a

Experimental models: organisms/strains

Mouse: C57BL/6JNarI National Laboratory Animal Center RMRC11005

Software and algorithms

FlowJo 10.6.0 FlowJo, LLC-BD Biosciences https://www.flowjo.com/

∗Alternative antibodies can be obtained from other manufacturers if generated from the same clones (i.e., from eBioscience).