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Potassium-gluconate based intracellular solution composition (pH 7.3, 290–300 mOsm/L):
| Reagent | Concentration (mM) | Mass/volume |
|---|---|---|
| K-gluconate | 142 | 1.6628 g |
| HEPES | 10 | 0.1191 g |
| KCl | 4 | 0.0149 g |
| MgCl2 (1 M stock) | 2 | 100 μL |
| Na2ATP | 2 | 0.0551 g |
| Na2Phosphocreatine | 1 | 0.0128 g |
| EGTA | 0.5 | 0.0095 g |
| Na2GTP | 0.3 | 0.0078 g |
| Biocytin | 2.7 | 0.0500 g |
| ddH2O | - | adjust to 50 mL total volume |
