Phosphate buffer 0.4 M stock solution composition (pH 7.4):
| Reagent | Concentration (mM) | Weight (g) |
|---|---|---|
| NaH2PO4 | 300 | 6.2 g |
| Na2HPO4 | 100 | 22 g |
| ddH2O | - | adjust final volume to 500 mL |
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