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. 2021 Feb 17;1154:338330. doi: 10.1016/j.aca.2021.338330

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Fig. 3 — Absorption spectra of reaction system containing different concentrations of DNA probes upon the addition of 0.1 × SSC (a), 0.2 × SSC (b), 0.3 × SSC (c), 0.4 × SSC (d), respectively. inset: colorimetric changes of AuNPs/DNA/SSC. Raman spectra (e) and fluorescence spectra (f) of AuNPs containing different concentrations of DNA probes upon the addition of 0.3 × SSC. (g) Absorbance spectra and (h) A_690nm/A_520nm of reaction system under different incubation time between probe DNA (0.05 μM) and AuNPs. (i) Absorbance spectra, (j) A_690nm/A_520nm, (k) fluorescence intensity, and (l) Raman spectra of detection system under different reaction time between target RNA (0.01 μM) and probe DNA. Parameter optimization experiments are completed at room temperature.