Figure 3.

ICD effect of supramolecular nanoparticles‐mediated PDT in the Panc02 cells in vitro. a) CLSM images of cellular uptake of HCJSP post‐24 h incubation (scale bar = 50 µm); b) Flow cytometry detection of cellular uptake of HCJSP pretreated with or without anti‐CD44 antibody; c) Photoactivity of HCJSP in vitro under 671 nm laser irradiation evaluated by CCK8 assay after 24 h treatment; d) Flow cytometry examination determined intracellular ROS generation; e,f) CLSM examination of PDT‐induced surface CRT expression and nuclear HMGB1 efflux in the Panc02 cells in vitro (scale bar = 50 µm); g) The flow cytometric analysis of PDT‐induced CRT exposure of Panc02 cells. Tumor cells were pretreated with HCP or HCJSP at a PPa concentration of 5.0 µm and received 671 nm laser irradiation at 60 mW cm⁻² for 30 s; h) Flow cytometry detection of PDT‐triggered BMDC maturation in vitro (mean ± s.d, *p < 0.05, **p < 0.01, ***p < 0.001).