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. 2021 Jan 4;8(4):2002746. doi: 10.1002/advs.202002746

Figure 5.

Figure 5

HCJSP‐mediated combinatory immunotherapy of pancreatic cancer. a) Experimental schedule for photoimmunotherapy of HCJSP; b,c) Representative flow cytometric plots of, b) intratumoral infiltrating CD4+ and CD8+ T cells, and c) intratumoral infiltrating IFN‐γ + CD8+ T cells. The tumor‐bearing mice were sacrificed 7‐days post‐treatment for flow cytometric analysis; d) matured DC in the tumor‐draining LNs; e) tumor mass normalized intratumoral infiltration of CD8+ T cells to CD4+ T cells ratios; f) CD8+ T cells to Tregs ratios in TILs; g) the frequency of memory CD8+ T lymphocyte in the spleen after 21‐days of treatment; h) intracellular IFN‐γ secretion determined by ELISA assay; i–k) IHC examination of i) HK‐2, j) LDHA, and k) PD‐L1 expressions in the tumor tissue at the end of antitumor study (scale bar = 200 µm); l) lactate secretion in the tumor tissue (mean ± s.d, *p < 0.05, **p < 0.01, ***p < 0.001).