Table 1.
Antibodies to sort hESC-derived cardiac subpopulations via FACS
| Cell surface or intracellular antigen | Company | Catalog number | Dilution | |
|---|---|---|---|---|
| Primary antibody | ||||
| Cardiac troponin T/TNNT2 FITC | intracellular | Abcam | ab105439 | 1:200 |
| CD31 APCCY7 | cell surface | BD Biosciences | 563653 | 1:150 |
| HCN4 PE | cell surface | StreeMarq Biosciences | SMC-320D-R-PE | 1:200 |
| ISL1 | intracellular | DSHB | 39.4D5 | 1:100 |
| ISL1 PE | intracellular | BD Biosciences | 562547 | 1:100 |
| PDGFRα Alexa Fluor (AF) 647 | cell surface | BD Biosciences | 562798 | 1:100 |
| SMMHC | intracellular | Biomedical Technologies | BT-562 | 1:20 |
| OCT3/4 | intracellular | Santa Cruz | sc-5279 | 1:50 |
| Targeted primary antibody | Company | Catalog number | Dilution | ||
|---|---|---|---|---|---|
| Secondary antibody | |||||
| Goat anti-mouse IgG2b secondary antibody, AF 647 | ISL1 (39.4D5) | Thermo Fisher Scientific | A-21242 | 1:1,000 | |
| Goat anti-rabbit IgG (H+L) secondary antibody, AF 405 | SMMHC (BT-562) | Thermo Fisher Scientific | A-31556 | 1:500 | |
| Goat anti-mouse IgG2b secondary antibody, AF 488 | ISL1 (39.4D5), OCT3/4 (sc-5279) | Thermo Fisher Scientific | A-21141 | 1:1,000 | |
| Isotype control | |||||
| Mouse IgG1 FITC | TNNT2 FITC (ab105439) | Abcam | Ab91356 | 1:20 | |
| Mouse IgG1 APCCY7 | CD31 APCCY7 (563653) | BD Biosciences | 557873 | 1:150 | |
| Mouse IgG1 PE | HCN4 PE (SMC-320D-R-PE) | Thermo Fisher Scientific | 12-4714-42 | 1:20 | |
| Mouse IgG2b | ISL1 (39.4D5) | Thermo Fisher Scientific | 14-4732-82 | 1:100 | |
| Mouse IgG1 PE | ISL1 PE (562547) | BD Biosciences | 554680 | 1:50 | |
| Mouse IgG2a AF 647 | PDGFRα AF647 (562798) | BD Biosciences | 557715 | 1:100 | |
| Rabbit IgG | SMMHC (BT-562) | Thermo Fisher Scientific | 02-6102 | 1:20 | |
| Mouse IgG2b AF 488 | OCT3/4 (sc-5279) | Santa Cruz | sc-3892 | 1:50 | |