Fig. 3.

Impaired EDR in HMGB1ECKO mice. A, Blood pressure including systolic, mean, and diastolic blood pressures from male control and HMGB1ECKO mice at three months was measured using the noninvasive computerized tail cuff system (n = 10). *P < 0.05 vs. control. B, Response to sodium nitroprusside in mice aortas. Vessels were precontracted with phenylephrine. All values are mean ± SD. C, Relaxation response to acetylcholine of aortas obtained from control and HMGB1ECKO mice with or without l-NAME. D, response to phenylephrine of aortas from control and HMGB1ECKO mice. E, representative response curve of aortas from control and HMGB1ECKO mice. F, Relaxation response to acetylcholine of aortas from HMGB1ECKO mice with ad-HMGB1 transfection or ad-Vector transfection. G, I, Representative Western Blot and quantitative analysis of HMGB1 expression in aortas from ad-HMGB1 or ad-Vector group. H, IF stain of HMGB1 in aortas transfected with or without ad-HMGB1 from HMGB1ECKO mice. (HMGB1, red; DAPI, blue; CD31, white; scale bars, 50 μm, 100 μm for enlarged window). Data are the average of triplicates from single experiments that were independently repeated 3 times. Comparisons were performed by using two-way ANOVA. (*P < 0.05). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)