Fig. 6.

SOD rescued endothelial dependent EDR and angiogenesis. A, EDR was tested in aortas from HMGB1ECKO mice with or without pre-incubation of PEG-SOD. B, HMGB1ECKO mice were injected with Ad-GFP or Ad-SOD2 and SOD2 levels examined in the adductor muscle group by Western Blot after 4 days of infection (C). Ad-SOD2 improved the time course of blood flow recovery (D). HUVECs transfected with HMGB1 siRNA were treated with or without PEG-SOD (100 μg/ml), then proliferation(G) and tube formation were tested. (E, F) Representative images were shown. Comparisons were performed by using two-tailed unpaired Student's t-tests for C, one-way ANOVA for F, G and two-way ANOVA for A, D. (n = 4–6, *P < 0.05).