Figure 1.

Ovine fibroblasts produced more homogeneous CAM sheets than canine or porcine fibroblasts. Macroscopic views of sheets produced in 6-well plates, and closer views of one well after 8 weeks of culture. CAM sheets were produced in cultures supplemented with 20% of two serums (S1 and S2) used either pure or in combinations (S1 5% + 15% S2, S1 10% + 10% S2, and S1 15% + 5% S2). (a–e) Human cells produced dense CAM sheets in all conditions, (f–j) canine cells produced non-homogeneous sheets that frequently tore and were not recoverable, (k–o) porcine cells produced very thin sheets in some conditions, (p–t) ovine cells produced the best-looking sheets of all animals’ cells.

Detachments (*) of canine (f–j) and ovine (q–t) sheets from the stainless-steel wire anchor were common but did not occur with human sheets (a–e). Scale bars = 35 mm.