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Human fibroblasts produced a denser ECM than ovine fibroblasts. Histological cross sections of multiple layers of human (a–e) and ovine (f–j) CAM sheets after 4, 5, 6, 7, and 8 weeks of culture (Masson’s trichrome, cells = purple, collagen = blue/green). Human and ovine cells were distributed throughout the sheet (red arrows) and a clear layer of cells was located on the top side of the sheet (*). Scale bar = 50 µm.
