Figure 7.

Silencing of FGD5-AS1 inhibited macrophages M2 polarization. (A) Exosomes isolated from supernatants of A549 cells were detected by transmission electron microscopy (TEM). Scale bar = 200 nm; (B) Western blot analysis of exosomes markers TSG101, CD63, and CD81; (C) The mRNA expression of macrophage marker CD68 was measured by qRT-PCR. ^**P < 0.01 vs. THP-1; (D) The expression of FGD5-AS1 was measured by qRT-PCR in the exosomes isolated from the BEAS-2B, A549, and H1299 cells. **P < 0.01 vs. BEAS-2B; (E) QRT-PCR was performed to confirm the expression of FGD5-AS1 in exosomes isolated from A549 cells. ^**P < 0.01 vs. sh-NC; (F) The mRNA expression of M2 macrophages markers CD163, CD206, and arginase 1 (ARG1) was measured by qRT-PCR in macrophages. ^*P < 0.05 vs. Control, ^##P < 0.01 vs. Exo-sh-NC; (G) The mRNA expression of M1 macrophage markers iNOS and interleukin 12 (IL-12) was detected by qRT-PCR in macrophages. ^*P < 0.05 vs. Control, ^##P < 0.01 vs. Exo-sh-NC.