Table 3:

SMARCA4 mutations in DUS

n	Age	SMARCA4 mutation	Predicted Origin	Mutation Type	Previously Identified
1	48	p.Ser127fs*174 p.Gln941*	Unknown	Frameshift Nonsense	No
2	51	Loss	Unknown	Loss	No
3	63	p.Asn175fs*105 p.Gln1053fs*53	Somatic	Frameshift Frameshift	No
4	52	p.Arg1093*	Indeterminate	Nonsense	Yes
5	43	p.LysK443fs*54 p.Glu1292fs*14	Somatic	Frameshift Frameshift	No
6	51	p.Gln1185*	Indeterminate	Nonsense	No
7	60	p.Arg1491*	Indeterminate	Nonsense	No
8	49	p.Gln201fs*102 p.Phe947fs*3	Indeterminate	Frameshift Frameshift	No
9	33	p.Tyr862*	Indeterminate	Nonsense	No
10	70	c.2505+1G>A	Somatic	Splice site	No
11	52	p.Gly256*	Indeterminate	Nonsense	No
12	44	p.Lys587fs*26	Somatic	Frameshift	No
13	38	p.Gly883fs*4 c.3236C>T	Indeterminate	Frameshift Splice site	No
14	55	p.Gln611* p.Lys1602fs*8	Germline	Nonsense Frameshift	No
15	45	c.1593+1G>A p.Glu1310*	Indeterminate	Splice site Nonsense	No
16	32	p.Lys578fs*35	Unknown	Frameshift	No
17	40	Loss c.3951+2_3951+20>AG	Germline	Loss Splice site	No