FIGURE 1.

SZB120 selectively inhibits Th17 cell differentiation. (A) The synthesis of SZB120. (B) Mouse primary naive CD4⁺ T cells from IL-17A–GFP mice were differentiated under Th17 cell polarization conditions for 72 h with DMSO and different concentrations of AβBA (0.5, 1, 2, 5 μM) or SZB120 (0.5, 1, 2, 5 μM) (n = 3). (C) Naive CD4⁺ T cells were differentiated under Th17 polarization conditions for 72 h with DMSO and SZB120 (0.5, 1, 2, 5 μM). CD4⁺ annexin V⁺ 7AAD⁺ cells were analyzed. The black bar represents the percentage of annexin V⁺ 7AAD⁻ cells (early apoptosis). The red bar indicates the percentage of annexin V⁺ 7AAD⁺ cells (late apoptosis) (n = 3). (D) The proliferation marker Ki67 was tested under the Th17 cell differentiation conditions mentioned above with DMSO and SZB120 (0.5, 1, 2, 5 μM). Cells were gated for CD4⁺ and stained for Ki67. Black bar indicates the mean fluorescence intensity (MFI) of Ki67⁺ cells (n = 3). (E) T cells were activated under Th1, Th2, and iT_reg cell polarization conditions in the presence of DMSO and 0.5, 1, 2, and 5 μM SZB120 (n = 3–6). Data are the mean ± SEM by two-way ANOVA (B and C) or one-way ANOVA (D and E) with Bonferroni correction for multiple comparisons. *p < 0.05, ***p < 0.001, compared with DMSO. ns, no significance