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. 2021 Jan 22;206(5):953–962. doi: 10.4049/jimmunol.2000036

FIGURE 4.

FIGURE 4.

SZB120 affects glucose uptake and glycolysis in Th17 cells. (A) Representative flow cytometric analysis of Th17 cells treated with DMSO or SZB120 (2 or 5 μM). Cells were collected for 100 μM 6-NBDG dilution at the indicated times (n = 3). (B) The effect of SZB120 on glycolysis in Th17 cells was evaluated by monitoring the ECAR using an XF96 Extracellular Flux Analyzer. Glucose (10 mM), the oxidative phosphorylation inhibitor (oligomycin 1 μM), and the glycolytic inhibitor 2-deoxy-glucose (2-DG, 50 mM) were sequentially injected into each well at the indicated time points (n = 3–5). Data are the mean ± SEM by one-way (A) or two-way ANOVA (B) with Bonferroni correction for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001 compared with DMSO.