Table 3. Intraday and Interday Precision, Accuracy, and Matrix Effect of Aconitum Alkaloids (n = 6).
| interday |
intraday |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| alkaloid | concentration (ng/mL) | precision (%) | RSD (%) | precision (%) | RSD (%) | recovery (%) | RSD (%) | matrix effect (%) | RSD (%) |
| AC | LLOQ | 95.3 | 9.25 | 108 | 5.98 | 95.6 | 6.34 | 95.4 | 13.3 |
| 10 | 97.6 | 10.30 | 97.5 | 12.6 | 88.7 | 5.98 | 98.6 | 5.64 | |
| 100 | 113 | 4.84 | 109 | 5.64 | 108 | 12.3 | 106 | 8.73 | |
| 500 | 86.7 | 12.6 | 107 | 8.79 | 96.8 | 11.8 | 100 | 9.51 | |
| HA | LLOQ | 93.8 | 11.8 | 96.8 | 6.67 | 97.6 | 13.3 | 114 | 12.6 |
| 10 | 106 | 9.78 | 97.6 | 13.5 | 106 | 9.64 | 109 | 11.0 | |
| 100 | 94.7 | 13.7 | 103 | 10.9 | 114 | 8.75 | 97.5 | 8.86 | |
| 500 | 85.4 | 14.3 | 86.1 | 7.29 | 106 | 7.94 | 86.4 | 9.74 | |
| MA | LLOQ | 112 | 10.8 | 100 | 4.65 | 87.6 | 8.47 | 105 | 9.56 |
| 10 | 109 | 7.43 | 95.3 | 3.35 | 107 | 6.85 | 96.1 | 4.85 | |
| 100 | 113 | 6.59 | 87.5 | 12.5 | 94.3 | 9.59 | 102 | 6.59 | |
| 500 | 98.7 | 12.8 | 106 | 13.8 | 87.6 | 5.76 | 97.3 | 7.35 | |
| BAC | LLOQ | 97.5 | 11.9 | 109 | 6.45 | 85.7 | 13.4 | 98.6 | 6.63 |
| 10 | 96.3 | 4.65 | 102 | 5.73 | 102 | 9.48 | 88.4 | 11.7 | |
| 100 | 86.4 | 7.85 | 91.6 | 11.9 | 104 | 8.72 | 108 | 13.4 | |
| 500 | 88.6 | 8.64 | 87.1 | 12.5 | 90.5 | 12.6 | 94.3 | 9.53 | |
| BHA | LLOQ | 93.5 | 10.3 | 109.3 | 9.58 | 86.6 | 9.84 | 96.2 | 8.51 |
| 10 | 91.4 | 9.42 | 92.9 | 6.75 | 94.8 | 7.85 | 99.5 | 4.73 | |
| 100 | 94.8 | 8.27 | 108.5 | 10.9 | 96.7 | 4.94 | 104 | 3.67 | |
| 500 | 96.1 | 11.3 | 107.6 | 8.60 | 87.6 | 13.8 | 89.9 | 10.8 | |
| BMA | LLOQ | 95.7 | 5.25 | 112.6 | 13.6 | 95.8 | 3.68 | 95.4 | 12.6 |
| 10 | 86.5 | 6.37 | 107 | 12.5 | 89.8 | 12.7 | 109 | 4.73 | |
| 100 | 107 | 13.3 | 96.4 | 7.65 | 108 | 5.84 | 97.6 | 6.82 | |
| 500 | 93.2 | 10.5 | 94.8 | 13.8 | 110 | 6.72 | 103 | 5.84 | |