Table 1. Identification and Quantification of Active MMPs in Mouse Woundsa.
| protein | peptide sequence | Q1 precursor ion m/z | Q3 product ion m/z |
|---|---|---|---|
| MMP-8 | QYWALSGYDLQQGYPR | 972.97 [M + 2H]2+ | 1196.57 [M + H]+y10 |
| 1139.55 [M + H]+ y9 | |||
| 976.48 [M + H]+y8 | |||
| DISNYGFPR | 534.76 [M + 2H]2+ | 840.40 [M + H]+y7 | |
| 753.37 [M + H]+y6 | |||
| 639.32 [M + H]+ y5 | |||
| TYFFINNQ[C]WR | 774.86 [M + 2H]2+ | 1137.53 [M + H]+ y8 | |
| 990.46 [M + H]+ y7 | |||
| 877.37 [M + H]+y6 | |||
| MMP-9 | DMIDDAFAR | 527.24 [M + 2H]2+ | 807.40 [M + H]+ y7 |
| 694.32 [M + H]+y6 | |||
| 579.29 [M + H]+ y5 | |||
| AFAVWGEVAPLTFTR | 832.94 [M + 2H]2+ | 1090.59 [M + H]+y10 | |
| 904.53 [M + H]+ y8 | |||
| 734.42 [M + H]+ y6 | |||
| GSPLQGPFLTAR | 622.34 [M + 2H]2+ | 889.49 [M + H]+ y8 | |
| 761.43 [M + H]+y7 | |||
| 704.41 [M + H]+ y6 | |||
| Internal Standard | TAGIQIVADDLTVTNPK | 878.48 [M+2H]2+ | 1172.62 [M + H]+y11 |
| 1002.51 [M + H]+ y9 | |||
| 772.46 [M + H]+ y7 | |||
| VNQIGTLSESIK | 644.86 [M + 2H]2+ | 1075.60 [M + H]+y10 | |
| 777.44 [M + H]+ y7 | |||
| 676.39 [M + H]+ y6 | |||
| NVNDVIAPAFVK | 643.86 [M + 2H]2+ | 844.53 [M + H]+ y8 | |
| 632.38 [M + H]+y6 | |||
| 561.34 [M + H]+ y5 |
a
Quantification was done using three peptides per MMP. Precursor m/z ions were selected in the first quadrupole (Q1), then fragmented in Q2. The specified fragment was monitored in Q3. The fragments used as “quantifier” are represented in bold and were assigned using conventional peptide fragmentation nomenclature. [C] = alkylated cysteine or carbamidomethyl cysteine.