Figure 4.

Evolution of 1⁴⁺ resistance of E. coli strain EC958 in glucose defined minimal media (GDMM). E. coli cultures were grown in liquid GDMM containing 0.5 × MIC of 1⁴⁺, cultures were passaged every 24 h and weekly MIC’s were determined. Experiments were performed as biological duplicates and results displayed are weekly mean MIC results (N ≥ 3 ± SD for each biological repeat). Control samples (upward and downward facing triangles) were treated the same as test samples (●, □), except no compound was added. Two-way ANOVA with Tukey’s multiple comparison test showed significant differences between the original WT cultures and both test cultures by week five and between the test and control cultures in week five (p < 0.0001).