Figure 6. Pggt1b shapes chromatin accessibility and AP-1 transcriptional programming.

(A) Principal component analysis plot of nucleosome-free reads of cT_reg and eT_reg cells from WT or Foxp3^CrePggt1b^fl/fl mixed bone marrow chimeras. (B) Similarity matrix plot of indicated cell populations as in (A). (C) Odds ratio/odds ratio plot of the predicted enriched motifs in Foxp3^CrePggt1b^fl/fl vs. WT eT_reg cells compared with Foxp3^CrePggt1b^fl/fl vs. WT cT_reg as in (A). (D) Binding profiles of Jun family transcription factors in WT or Foxp3^CrePggt1b^fl/fl eT_reg cells as in (A). (E) Quantification of CD5 or Nur77-GFP expression in Foxp3-RFP⁺ cT_reg cells activated by anti-CD3/28 plus IL-2 (or IL-2 alone, black bars) in the presence or absence of GGTI for 24 h. (F) Quantification of conjugate formation of Foxp3-YFP⁺ cT_reg or eT_reg cells from WT or Foxp3^CrePggt1b^fl/fl mice co-cultured with Celltrace Violet-labeled DCs in the presence of IL-2 for 10 h. (G) Flow cytometry analysis and quantification of the frequency of Foxp3⁺YFP⁺ T_reg cells from WT or Foxp3^Cre/wtPggt1b^fl/fl ‘mosaic’ mice found in the red pulp/marginal zone(RP/MZ; CD4-RM4–5⁺CD4-RM4–4-PE⁺) or white pulp (WP; CD4-RM4–5⁺CD4-RM4–4-PE⁻).

Data are representative of two (E), or compiled from two (F, G) independent experiments, with three (E, G) or at least two (F) biological replicates per group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; two-tailed unpaired Student’s t-test (F, G) or one-way ANOVA (E). Data are mean ± s.e.m. Numbers indicate percentage of cells in gates. Controls were normalized to 1 for each comparison (black bars). See also Figure S6.