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Design of tripeptide probes and the assay to assess acyl-acceptor strand in live cells. (a) Structure-based design of acyl-donor and -acceptor probes. (b) Schematic diagram showing how the fluorescently tagged tripeptide probe gets covalently crosslinked into the bacterial PG scaffold. (c) Schematic of the whole cell analysis that can be accessed by the tripeptide probes.
