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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: ACS Chem Biol. 2020 Apr 3;15(5):1261–1267. doi: 10.1021/acschembio.0c00002

Figure 3.

Figure 3.

Crosslinking of tripeptide probes in live E. faecium cells. (a) Chemical series tripeptide probes based on the modifications at the d-iGlx and lysine sidechain; FAM = carboxyfluorescein. Flow cytometry analysis of E. faecium (D344s) treated overnight with 100 ¼M of tri-peptide probes. Data are represented as mean + SD (n = 3). (b) Confocal microscopy of E. faecium cells labeled with the designated probes (100 μM) for 5 min. Scale bar = 2 μm. (c) Labeling of cells similar to (a) with a focused library of re-arranged crossbriges.