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. 2021 Feb 16;21:60. doi: 10.1186/s12890-021-01420-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — PFKFB4 interacted with SRC-2 in lung adenocarcinoma cells. a, b Expression of PFKFB4 and SRC family was analyzed among 515 primary tumors and 59 normal samples in lung adenocarcinoma (LUAD) via UALCAN Platform (http://ualcan.path.uab.edu/index.html). c 293 T cells expressing myc-PFKFB4 was transfected with three SRC family members (SRC-1, SRC-2 or SRC-3) respectively. Myc–PFKFB4 was immunoprecipitated followed by western blot to detect the interaction status of SRC family proteins. pCMV6-Myc was used as a negative control. d 293 T cells expressing HA-SRC-2 was transfected with empty vector or myc-PFKFB4. HA-SRC-2 was immunoprecipitated followed by western blot to detect the interaction with PFKFB4. pCMV6-HA was used as a negative control. e, f Endogenous immunoprecipitation was performed in A549 and NCI-H1975 cells with or without anti-PFKFB4 antibody. The interacted endogenous SRC-2 was detected by western blot. IgG was used as a negative control