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. 2021 Feb 16;21:60. doi: 10.1186/s12890-021-01420-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — PFKFB4 phosphorylated SRC-2 at Ser487. a, b The knockdown efficiency of shPFKFB4 (#1 and #2) in A549 and NCI-H1975 was determined by western blot. GAPDH served as an internal control. c, d SRC-2 expression was detected by western blot in A549 and NCI-H1975 expressing shNC and shPFKFB4 (#1 and #2). GAPDH served as an internal control. e, f The phosphorylation of SRC-2 at Ser469, Ser487, Ser493, Ser499 and Ser736 in A549 and NCI-H1975 expressing shPFKFB4 (#1 and #2) was detected by western blot. g Endogenous immunoprecipitation was performed in A549 and NCI-H1975 cells with or without anti-PFKFB4 antibody. The phosphorylated SRC-2 at Ser487 was detected by western blot. IgG was used as a negative control. (H) Co-immunoprecipitation between PFKFB4 and SRC-2 WT or the phosphor-deficient SRC-2 mutant (SRC-2 S487A) was performed in A549 cells