Fig. 6.

PFKFB4 promoted lung adenocarcinoma cells proliferation, migration and invasion by regulating SRC-2/CARM1. a CARM1 expression at mRNA level was examined in A549 and NCI-H1975 transfected with sh-PFKFB4, CARM1, sh-PFKFB4 + CARM1 or control by qRT-PCR. *P < 0.05; **P < 0.01. All experiments are performed in triple. b CARM1 expression at protein level was examined in A549 and NCI-H1975 transfected with sh-PFKFB4, CARM1, sh-PFKFB4 + CARM1 or control by western blot. c CCK-8 assay was performed among A549 and NCI-H1975 transfected with sh-PFKFB4, CARM1, sh-PFKFB4 + CARM1 or control. *P < 0.05; **P < 0.01. All experiments are performed in triple. d, e Transwell and Transwell-matrigel assay were performed among A549 and NCI-H1975 transfected with sh-PFKFB4, CARM1, sh-PFKFB4 + CARM1 or control. Representative images are captured with the magnification of × 200. **P < 0.01. All experiments are performed in triple