Table 3. Validation of Oleocanthal-Derived Compounds at 37 °C for 2 h.
| A. Mean (±SD) Linear Regression Equations of the Two Formed Compounds | ||||
|---|---|---|---|---|
| biological matrix | calibration range (μg/mL) | compound | equation | R2 |
| Culture medium | 5–200 | Oleoglycine | y = 672 (±3)x + 278 (±67) | 0.9992 |
| 5–200 | Tyrosol acetate | y = 739.21 (±8)x – 1771 (±200) | 0.9955 | |
| Mouse plasma | 1.25–100 | Oleoglycine | y = 1333 (±62)x – 894 (±13) | 0.9951 |
| 1.25–100 | Tyrosol acetate | y = 1004 (±109)x – 863 (±332) | 0.9942 | |
| Mouse brain | 1.25–50 | Oleoglycine | y = 716 (±15)x – 868 (±119) | 0.9984 |
| Homogenate | 1.25–50 | Tyrosol acetate | y = 3781 (±32)x + 72 (±87) | 0.9948 |
| B. Intra- and Interday Precision Presented as Coefficient of Variation (CV%, n = 5/concentration) | ||||
|---|---|---|---|---|
| intraday
precision (CV %) |
interday
precision (CV%) |
|||
| oleocanthal concentration (μg/mL) | oleoglycine | tyrosol acetate | oleoglycine | tyrosol acetate |
| Culture medium | ||||
| 2.5 | 7.0 | 4.4 | 10.4 | 12.7 |
| 5 | 8.0 | 6.7 | 5.2 | 1.4 |
| 100 | 2.1 | 3.6 | 4.4 | 0.3 |
| Mouse plasma | ||||
| 5 | 9.4 | 3.1 | 0.1 | 4.0 |
| 25 | 9.9 | 3.2 | 7.7 | 4.7 |
| 50 | 3.9 | 6.5 | 8.8 | 3.4 |
| C. Freeze and Thaw Stability of Oleocanthal (n = 4/Concentration) Presented as % Difference to Freshly Prepared Samples | ||||
|---|---|---|---|---|
| culture
medium (%) |
mouse
plasma (%) |
|||
| oleocanthal concentration (μg/mL) | oleoglycine | tyrosol acetate | oleoglycine | tyrosol acetate |
| 2.5 | 84.5 | 87.9 | 113.0 | 103.0 |
| 25 | 107.9 | 94.4 | 97.1 | 90.5 |
| 50 | 98.5 | 104.7 | 111.4 | 97.7 |