Figure 8.

Tight junction proteins and eNOS levels are regulated by calpain inhibitors. Quantitative analysis of immunoblots of ipsilateral hemispheres of WT and ALDH2^–/– mice 24 h post-mTBI with or without 5 or NYC-438 (10 mg/kg i.p.) and probed with ZO-1 (A), occludin (B), claudin-5 (C), and eNOS (D). Data represent mean ± SD of n = 7–10 animals analyzed by one-way ANOVA with Tukey’s multicomparison analysis. All protein was normalized to the housekeeping protein, β-actin; 50 μg of protein was loaded in each well. *, p < 0.05; **, p < 0.01; and ***, p < 0.001.