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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Br J Haematol. 2019 Jul 15;187(4):488–501. doi: 10.1111/bjh.16092

Figure 2. Anti-proliferative effects of BLU-285 and FLT3 inhibitors on growth of Ba/F3-KIT-D816V cells, and effects of FLT3 inhibitors versus BLU-285 on phosphorylation of KIT.

Figure 2.

(A) Anti-proliferative effects of BLU-285 and FLT3 inhibitors (100 nM) on growth of Ba/F3 and Ba/F3-KIT-D816V cells following 3 days of treatment. (B) Proliferation studies: Growth curves showing drug treatment effects on Ba/F3 or Ba/F3-KIT-D816V cells following 3 days of treatment. (C,D) Immunoblots: Measurement of drug effects on phosphorylation of KIT in Ba/F3-KIT-D816V cells following 2 hour treatments with inhibitors at a concentration of 200 nM. Phospho-KIT (Y719) antibody was used for (C) and phospho-KIT (Y703) antibody was used for (D).