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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Br J Haematol. 2019 Jul 15;187(4):488–501. doi: 10.1111/bjh.16092

Figure 5. Effects of FLT3 inhibitors versus BLU-285 on phosphorylation of FLT3 in FLT3-ITD-expressing MOLM14 cells and on phosphorylation of KIT in N822K-expressing Kasumi-1-luc+ cells.

Figure 5.

(A-C) Immunoblots: Measurement of drug effects on phosphorylation of FLT3 in MOLM14 cells (A) and on phosphorylation of KIT in Kasumi-1-luc+ cells (B, C) following 2 hour treatments with inhibitors at the indicated concentrations. Phospho-KIT (Y719) antibody was used for B and C. (D) KIT immunoprecipitation/pTYR immunoblot for crenolanib (100-200 nM) -treated Kasumi-1; as positive control, FLT3 immunoprecipitation/pTYR immunoblot for crenolanib (100-200 nM)-treated MOLM14.