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. 2021 Feb 16;19:31. doi: 10.1186/s12915-021-00969-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Proboscis event duration changes with various parameters in a choice assay. a Event analysis from Fig. 5e, 15 min, reveals that proboscis event duration increases with food deprivation on the low-current well only (For this figure, medians with 95% confidence intervals are shown. For the analysis, the data were log-transformed to run 2-way ANOVAs, see the “Methods” section. Main effect of food well, p = 0.0006, but not of deprivation time, p = 0.41; event duration is no different between the high- vs. low-current well after 6-h food deprivation, p = 0.23, but longer on the low-current 20-MΩ well after 18 h, **p = 0.0016, Sidak’s multiple comparison test, n = 136, 75, 84, 116 events from 10 arenas per condition with 10 Canton-S males). b Analysis of Fig. 5f, 15 min, shows a main effect of well (p = 0.041), deprivation (p = 0.0012), and interaction (p = 0.029, n = 239, 209, 331, 368 events from 19 to 20 arenas). There is a slight, but highly significant increase in proboscis event duration on the higher current/higher sucrose well (***p < 0.0001) compared to the lower current/lower sucrose after 18 h of food deprivation. c Event analysis from Fig. 4c reveals a main effect of the well (p = 0.019), sucrose concentration (p = 0.028), and interaction (p < 0.001). With equal sucrose, flies had longer event durations on the lower current well (***p < 0.001), but they had longer proboscis event durations on the high-current well when paired with higher, 400 mM sucrose (*p = 0.015). Event duration increased significantly with sugar concentration on the high-current/variable-sucrose well (p = 0.006, linear regression), and it decreased significantly on the low-current/constant-sucrose well (p < 0.001; n = 164, 247, 139, 223, 165, 155 events from 7 to 8 arenas per condition, 10 Canton-S males)