Figure 8.

RNA-sequencing analysis of Wnt and Wnt/β-catenin–regulated gene expression levels and stem cell PCR array analysis of control and RAD6B knockout (KO) M14 melanoma cells and normal HEMa-LP melanocytes. A: Heat map of WNT and β-catenin–regulated gene expression levels from RNA-sequencing analysis. B: RT2 Stem Cell real-time PCR analysis of control and RAD6B KO M14 cells. C: Pearson correlation analysis of genes identified by RT2 Profiler real-time PCR and RNA-sequencing analysis (R² = 91.11%; P < 0.001). The dotted line indicates the fitted linear regression line.