Figure 9.

The prevention of LTD in stressed mice does not influence the induction of LTD in naive partners. A, Schematic showing the experimental designs and timeline. Male adult mice were housed in pairs for at least 1 week. In the experiment, the subject mouse was removed, was received bilateral injections of vehicle (Veh; PBS) or APV (3.2 µg, 0.5 µl per side) in the dorsal hippocampus, was exposed to a restraint–tailshock stress paradigm for 90 min, and then returned to its home cage and allowed to freely interact with the naive partner for 30 min before slice preparation. B, Summary of experiments showing the grand mean LTD magnitudes at Schaffer collateral–CA1 synapses in slices from vehicle-treated stressed male mice and naive male partners. C, Summary of experiments showing the grand mean LTD magnitudes at Schaffer collateral–CA1 synapses in slices from APV-treated stressed male mice and naive male partners. D, Summary of experiments showing the induction of hippocampal CA1 LTD by LFS at Schaffer collateral–CA1 synapses in slices from vehicle-treated stressed male mice. E, Summary of experiments showing the induction of hippocampal CA1 LTD by LFS at Schaffer collateral–CA1 synapses in slices from naive male partners in pairing with vehicle-treated stressed mice. F, Summary of experiments showing the induction of hippocampal CA1 LTD by LFS at Schaffer collateral–CA1 synapses in slices from naive male partners in pairing with APV-treated stressed mice. G, Bar graph illustrating the proportion of individuals that express LTD or no LTD in naive partner and stressed mice received either Veh or APV treatment. Representative traces of fEPSPs were taken at the time indicated by number. Dashed lines show the level of baseline. Data represent the mean ± SEM.