Figure 2.

Patchwork-type spontaneous activity in control L4 neurons. A, Confocal image of a representative tangential section from a Supernova-GCaMP6s-transfected TCA-RFP; Grin1floxed/+ mouse, taken after in vivo imaging at P6. B, Time-lapse in vivo calcium imaging was performed in the same region as in A. C, ROIs are located on the cell bodies of neurons imaged in B. D, Changes in fluorescence signals in the ROIs indicated in C. E, Correlation matrix calculated from the fluorescence signals of all ROI pairs measured in C. F, Correlation values of ROI pairs of GluN1 heterozygous L4 neurons within the same barrel were higher than those in different barrels (p < 0.001). G, Distribution of correlation values for cell pairs from the same barrel and different barrels with respect to distance. H, Comparison of correlation values between ROI pairs from the same barrel and those from different barrels (total: p < 0.001; 0–200 µm: p < 0.001). Error bars indicate SE.