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. 2021 Jan 21;11:575255. doi: 10.3389/fmicb.2020.575255

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Copyright © 2021 Inagaki, Sato, Ito, Takaki, Okuno, Yaguchi, Masud, Watanabe, Sato, Iwami, Murata and Kimura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Profile of EBV gene expression in the pre-latent phase of EBV infection of primary B-cells. (A) Purified primary B-cells were infected with EBV-EGFP and harvested at 0, 2, 4, 7, or 14 dpi. EBV gene expression was assessed by qRT-PCR and normalized to GAPDH expression. Results are presented as means ± SD of three independent experiments and are shown relative to gene expression at 2 dpi. (B) Akata(–) or purified primary B-cells were infected with EBV-EGFP and harvested at 0 and 3 dpi. EBV gene expression was assessed by qRT-PCR and normalized to GAPDH expression. Results are presented as means ± SD of three independent experiments. Abbreviations: dpi, day post-infection.