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. 2021 Jan 21;11:575255. doi: 10.3389/fmicb.2020.575255

FIGURE 7.

FIGURE 7

Abortive lytic gene expression of BZLF1-KO recombinant EBV in the pre-latent phase. (A) Akata(–) cells were infected with wild-type, BZLF1-KO, or BMRF1-KO (BMRF1p-Flpe) EBV. After 2 days, total RNA was extracted and analyzed by qRT-PCR. Results are presented as means ± SD of three independent experiments and are shown relative to gene expression in the lytic-induced Akata(+) cells after treatment with IgG. (B) Purified primary B-cells were infected with wild type or BZLF1-KO EBV and harvested at 3 dpi. EBV gene expression was assessed by qRT-PCR and normalized to GAPDH expression. Results are presented as means ± SD of three independent experiments.